Master of Science in Biotechnology Program, UW-Madison

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Course Descriptions

Molecular Technologies III: Assay Development

Instructors:

  • Karin Borgh, Ph.D.
  • Natalie Betz, Ph.D.
  • This powerful exploration of drug-discovery topics and concepts puts students in the role of scientists researching an inhibitor of Src Protein Kinase as a new drug therapy.

    Hands-on laboratory sessions cover state-of-the-art assays, methods and data analysis used in primary and advanced levels of drug screening.

    You'll focus on detecting kinase activity as a model for a relevant drug target, evaluate the effect of two putative inhibitors identified from a compound library during an initial screen and assess how the identified inhibitors effects cytochrome P450 activity and cell viability.

    You'll gain experience with basic cell culture techniques, stem cell biology, fluorescent microscopy and high content screening. And as you master course concepts and lab practices, you'll also hone your scientific communication skills with short papers and a detailed scientific protocol.

    Here are some of the topics taught in this course:

    Session Topics from Fall 2019

    Session 1: Introduction to MTIII; Introduction to the Drug Discovery Process and Targets; Introduction to Kinase and P450 Enzymes and Assay Technologies; Thermo Fisher Drug Screening Service Laboratory Tour

    Session 2: Introduction to Data Analysis; Kinase Assay Laboratory; Introduction to the Final Capstone Project; Business Concepts Refresher

    Session 3: Cytochrome P450 Technology Laboratory and Introduction to Cell Culture and Cell-Based Assays

    Session 4: Introduction to Fluorescent Microscopy, High Content Screening, and In Vivo Imaging; Basic Cell Culture Laboratory Activities

    Session 5: Stem Cells: Introduction and Role in Drug Discovery

    Session 6: Cell-Based Assay Laboratory and Introduction to CRISPR

    Session 7: Final Presentations

    Question about the degree?
    Contact Bryan

    Bryan Husk
    Assistant Director
    Phone: (608) 265-0773
    bthusk@wisc.edu
    Contact Bryan Husk, Assistant Director, M.S. in Biotechnology Program, UW-Madison
    Class of 2020 Schedule

    Fall 2018 | Spring 2019
    Fall 2019 | Spring 2020

    Class of 2021 Schedule

    Fall 2019 | Spring 2020
    Fall 2020 | Spring 2021

    Class of 2022 Schedule

    Fall 2020 | Spring 2021
    Fall 2021 | Spring 2022